Part:BBa_Z0109

T7 gene 1.3

The ATP-dependent DNA ligase is only essential in E. coli lig mutants, but its presence enhances phage growth in wild-type hosts.

Usage and Biology

DNA ligase

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 652